Figure 2From: Homeobox transcription factor muscle segment homeobox 2 (Msx2) correlates with good prognosis in breast cancer patients and induces apoptosis in vitro Validation of Msx2 antibody specificity. (a) Western blot analysis of breast cell lines for Msx2 expression, showing Msx2 migrating at approximately 37 kDa. β-actin levels were used to evaluate protein loading. (b) Immunohistochemical staining for Msx2 on formalin-fixed, paraffin-embedded (FFPE) breast cell lines (×20 magnification). (c) Immunohistochemical staining for Msx2 on breast tumour tissue microarray (TMA) cores, showing examples of tumours with scores from 0 to 3 for nuclear and cytoplasmic staining (×20 magnification; scale bar represents 10 μm).Back to article page