Figure 5
Effect of NR4A1 on cell adhesion and integrin expression. (a) Control or MCF10A-NR4A1 cells were plated on wells coated with fibronectin, collagen type I, vitronectin or laminin I. Attached cells were fixed and stained with crystal violet. MCF10A-NR4A1 cells show increased adhesion to fibronectin and decreased adhesion to collagen type I compared with control cells, while both cell lines show similar adhesion levels to vitronectin and laminin I (mean ± standard deviation of six wells, *P < 0.001). (b) MCF10A-NR4A1 cells or control cells were analysed by flow cytometry for the cell surface expression of the integrins depicted. MCF10A-NR4A1 cells have increased levels of α5 and β4 integrins.