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Figure 4 | Breast Cancer Research

Figure 4

From: Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines

Figure 4

NR4A1 reduces the migratory ability of breast cancer lines. NR4A1 was ectopically expressed in three breast cancer lines (ZR-75-1, PMC42 and MDA-MB-231) and one normal mammary epithelial cell line (226L). (a) Ectopic expression was confirmed at the protein level by western blot. (b) The cell lines were then used for transwell migration assays where cells were allowed to migrate for 8 hours (226L and MDA-MB-231) or overnight (PMC42 and ZR-75-1) towards full culture medium. In all lines apart from MDA-MB-231, NR4A1 reduced the migratory ability of the cells. (c) The invasive lines PMC42 and MDA-MB-231 were used in transwell invasion assays. Cells were allowed to invade through the matrigel-coated transwells for 8 hours (MDA-MB-231) or overnight (PMC42). NR4A1 expression had no effect on the invasion of the tumour lines. Graphs show the mean ± standard deviation of one representative experiment out of n = 3. *P < 0.001. C, control; N, NR4A1.

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