Figure 3
Temporal protein expression of genes identified to respond to tamoxifen in vivo. MCM2, CKS2, IGFBP5 and TFF3 have similar expression at the protein level in response to tamoxifen in the ZR-75 xenograft by semi-quantitative immunohistochemistry. (a) They represent two pairs of examples of early/transiently and later/continuously responding proteins respectively. The correlation between the change in expression of proteins identified in the study and change in tumor volume in 28 patients treated with tamoxifen was calculated. Protein levels were scored by immunohistochemistry in tumor samples taken before and three months after treatment with tamoxifen. Changes in protein score are plotted relative to reduction in tumor volume for (b) IGFBP5 and (c) TFF3.