Figure 3

The role of PR in the P4-repressed epithelial-mesenchymal transition and cell proliferation. (a) Growth-arrested MB468 cells were treated with progesterone (P4; 30 ng/ml) or E2 (30 pg/ml) for 24 and 48 hours as indicated. (b) Growth-arrested MB468 cells were treated with R5020 (10 nM) or RU486 (10 nM) for two hours, followed by P4 treatment at 30 ng/ml for 24 hours. Western blot analyses were performed with anti-snail, anti-progesterone receptor (PR) and anti-α-tubulin antibodies. (c) MB468 cells were treated with R5020 (10 nM) or RU486 (10 nM) for two hours, followed by P4 treatment at 30 ng/ml for 24 hours. Cell proliferation assays were then performed. As shown in the Figures 3a to c, the P4 treatment inhibited snail expression and cell proliferation at equivalent levels. The results are representative for three separate experiments.