Skip to main content

Table 1 Relative expression of DNA repair and cell cycle proteins in MCF-7 monolayer and mammosphere populations

From: Senescence evasion by MCF-7 human breast tumor-initiating cells

Protein

Dose(Gy)

Normalized band intensity (n = 3)

P value

  

Monolayer

Mammosphere

 

APE1

0

1.00

2.10

0.024

 

1

1.02

1.92

0.052

 

10

1.07

1.84

0.017

ATM

0

1.00

1.99

0.018

 

1

1.13

2.23

0.011

 

10

1.62

1.99

0.088

p21

0

1.00

0.04

0.001

 

1

1.16

1.04

0.207

 

10

1.17

1.11

0.310

Chk1

0

1.00

2.17

0.003

 

1

1.03

2.06

0.018

 

10

1.05

2.31

0.052

phospho-Chk1

0

1.00

0.92

0.116

 

1

1.13

0.80

0.027

 

10

1.08

0.85

0.006

Chk2

0

1.00

2.39

0.001

 

1

0.92

2.46

0.001

 

10

0.93

2.51

0.005

phospho-Chk2

0

1.00

0.69

0.034

 

1

0.91

0.64

0.030

 

10

0.84

0.91

0.180

  1. APE1, apurinic/apyrimidinic endonuclease 1; ATM, ataxia telangiectasia mutated; phospho-Chk1, Chk1 phosphorylated at Ser345; phospho-Chk2, Chk2 phosphorylated at Thr68.