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Figure 1 | Breast Cancer Research

Figure 1

From: Breast cancer-specific mutations in CK1ε inhibit Wnt/β-catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration

Figure 1

Casein kinase 1 epsilon mutant phosphorylation of Dishevelled. All three mutants of casein kinase 1 epsilon (CK1ε) bind Dvl but are unable to cause the typical phosphorylation-dependent shift of the Dvl protein. (a) Schematic representation of the CK1ε mutants used in this study. (b) HEK293 cells were transfected with a plasmid encoding Dvl2-Myc and the CK1ε variants. CK1ε phosphorylates Dvl proteins, which subsequently exhibit a mobility shift in western blot analysis. The P6 mutant maintains residual activity, while the P3 and P4 mutants are unable to promote a Dvl2-Myc shift. (c) Truncated versions of CK1ε that lack the C-terminal autoinhibitory domain do not differ from full-length proteins in their ability to shift the Dvl2 protein.

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