Effect of PC-PLC inhibition on HER2 internalization in SKBr3 cells. CLSM analyses of SKBr3 cells cultured in complete medium either in the absence (t = 0) or presence of D609 (50 μg/mL) for the indicated incubation times. After washing, cells were fixed and stained with α-PC-PLC (green) and α-HER2 Abs (red). Nuclei were stained with DAPI (blue). Colocalization areas were represented in yellow (merge between green and red) or cyan (merge between green and DAPI). Scale bars, 8 μm. Panels show representative examples of five independent series of experiments.