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Figure 1 | Breast Cancer Research

Figure 1

From: Promotion of variant human mammary epithelial cell outgrowth by ionizing radiation: an agent-based model supported by in vitro studies

Figure 1

The growth of human mammary epithelial cells cultures exposed to 200 cGy exceeds that of unirradiated cultures. (A) Plots of the total population doublings of irradiated and unirradiated human mammary epithelial cells (HMEC) cultures versus days in culture for three experiments in which HMEC from different individuals were used (top to bottom; specimens B1450, B1400, and B1389). Each of the experimental points plotted in the graphs represents one passage. The time of irradiation between Passages 4 and 5 is indicated with a thunderbolt symbol. (B) Representative fields are seen in phase micrographs of unirradiated (U) and irradiated (IR) cultures at fourth passage (4p) 48 hrs after irradiation, or at fifth passage (5p) four to six weeks after irradiation. (C) Representative images of 5p plates stained with crystal violet illustrate the presence of larger and more frequent patches of actively growing cells in cultures derived from irradiated vs. unirradiated cells.

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