Human mammary cancer progression model recapitulates methylation events associated with breast premalignancy

Breast Cancer Research

Table 3 Description of normal, premalignant, and malignant breast tissues utilized for MSP analysis along with methylation status of RASSF1A and SFRP1 in each tissue

Sample	Description	RASSF1A	SFRP1
N1	Normal reduction mammoplasty tissue	U	U
N2	Normal reduction mammoplasty tissue	U	U
N3	Normal reduction mammoplasty tissue	M	M
N4	Normal reduction mammoplasty tissue	U	U
N5	Normal reduction mammoplasty tissue	U	U
N6	Normal reduction mammoplasty tissue	U	U
N7	Normal reduction mammoplasty tissue	U	U
Total Normal Methylated		1/7	1/7
H1	Lobular hyperplasia (85% of mammary cells), blunt duct hyperplasia (15% of mammary cells)	U	U
H2	Normal (80%), intraductal hyperplasia (10%), cystic hyperplasia (10%)	U	U
Total Hyperplasia Methylated		0/2	0/2
H3	Focal atypical terminal duct hyperplasia (single focus); fibrocystic disease	M	M
Total ADH Methylated		1/1	1/1
D1	CCL, DCIS	M	M
D2	DCIS	M	M
D3	DCIS, cysts, normal breast tissue	M	M
D4	DCIS and normal tissue	U	M
D5	Extensive comedo DCIS, calcifications	M	M
D6	Extensive DCIS	M	M
D7	Extensive DCIS and normal tissue	M	M
D8	DCIS, calcifications	U	M
D9	Extensive DCIS	M	M
D10	DCIS with calcifications	M	M
D11	Extensive comedo DCIS	M	M
Total DCIS Methylated		9/11	11/11
C1	IDC, grade 2, node-, ER+	M	M
C2	IDC, grade 3, node+, ER-	M	M
C3	IDC, grade 3, node+, ER-	U	M
C4	IDC, grade 3, node-, ER-	M	M
C5	IDC, grade 3, node+, ER+	M	M
C6	IDC, grade 1, node+, ER+	M	M
C7	IDC, grade 3, node+, ER-	M	M
C8	IDC, DCIS, CCL, normal breast	M	M
C9	IDC, DCIS	M	M
C10	IDC, DCIS, normal breast	U	M
C11	IDC and extensive DCIS	M	M
C12	IDC and extensive comedo DCIS with calcifications	M	M
Total IDC Methylated		10/12	12/12

ADH = atypical ductal hyperplasia; CCL = columnar cell lesion; DCIS = ductal carcinoma in situ; IDC = invasive ductal carcinoma; ER = estrogen receptor; U = unmethylated; M = methylated. For SFRP1, methylation status of the CpG island located in exon 1 is shown.

ISSN: 1465-542X