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Table 2 Methylation-specific PCR analysis of genes methylated in the human mammary epithelial cell progression model and in mammary cell lines

From: Human mammary cancer progression model recapitulates methylation events associated with breast premalignancy

 

HMEC

vHMEC

vector

ras

ras0.5

184-A1

MCF-10A

MCF-7

MDA-231

p16 INK4A

U

M

M

M

M

M

M

M

M

HOXA9

U

M

M

M

M

M

M

M

M

CCND2

U

M

M

M

M

M

M

M

M

RASSF1A

U

U

U

U

U/M

M

M

M

M

SFRP1*

U

U

U

U

M

U/M

U/M

M

M

p57

U

U

U

U

M

U

M

M

U

MGMT

U

U

U

U

U/M

U

U

U

U/M

THBS1

U

U

U

U

U

U

U

U

U

  1. SFRP1* Methylation status of the CpG island located in exon 1 is shown.