Figure 4

Vascular endothelial growth factor, vascular endothelial growth factor receptor 2 and hypoxia-inducible factor-1α expression in MCF7, Human umbilical vein endothelial cells and MDA-MB-231 respectively. A: MCF7 cells stained with pancytokeratin/FITC (green) and VEGF/Alexa555 (red) or MCF7 cells stained with pancytokeratin/FITC (green) and just Alexa555 antibody without VEGF antibody (negative control). B: HUVEC cells stained with pancytokeratin/FITC (green) and HIF1-α/Alexa555 (red) or HUVEC cells stained with pancytokeratin/FITC (green) and just Alexa555 antibody without HIF1-α antibody (negative control). C: MDA-MB-231 cells stained with pancytokeratin/FITC (green) and VEGFR2/Alexa555 (red) or MDA-MB-231 cells stained with pancytokeratin/FITC (green) and just Alexa555 antibody without VEGFR2 antibody (negative control).