Figure 4

Loss of p190B results in decreased tumor vasculature and fewer lung metastases. (a) Number of vessels per 100× section of tumor stained with von Willebrand factor (VWF) was quantified. Student's t test shows a significant difference between groups (P < 0.0001). (b) Representative pictures of VWF immunofluorescence (red) and dapi (blue) in the two different genotypes as indicated. Arrows mark blood vessels. Scale bar = 100 μm. (c) Number of metastases per mouse at tumor burden, as determined by counting metastatic nodules in serial sections of the entire lung. Student's t test showed a significant decrease in p190B^+/-Neu tumor mice (P < 0.03). Error bars, standard error of the mean.