Figure 3
From: The role of YY1 in reduced HP1α gene expression in invasive human breast cancer cells
YY1 is a positive regulator of HP1α expression. (a) MCF7 cells were transfected with an siRNA targeting luciferase (Luc) or ying yang 1 (YY1; 59, OT), and protein and RNA were extracted five days later. Western blot shows levels of YY1 and β-tubulin following knockdown with the indicated siRNA. (b) Relative mRNA levels were determined using quantitative RT-PCR. Quantitative RT-PCR results were normalized to the level of 28S ribosomal RNA. HNRPA1 = heterogeneous nuclear ribonucleoprotein A1.