Figure 1
From: The role of YY1 in reduced HP1α gene expression in invasive human breast cancer cells
HP1α expression is down-regulated in the invasive cell line HS578T compared with the non-invasive cell line MCF7. (a) A schematic of the human HP1α gene promoter region. The positions of transcription factor binding motifs as well as the 5' end of the CbxD98 luciferase reporter construct are noted. (b) The sequence of the proximal HP1α gene promoter region. Nucleotides in red are conserved (identical) between human, mouse, rat and chimpanzee. Transcription factor binding motifs are underlined. The 5' ends of the CbxU283 and CbxU146 luciferase reporter constructs are noted. (c) mRNA expression of HP1α, heterogeneous nuclear ribonucleoprotein A1 (HNRPA1), and ying yang 1 (YY1) in MCF7 and HS578T cells. Quantitative RT-PCR results were normalized to the level of 28S ribosomal RNA. (d) Western blots showing protein levels of HP1α, HNRPA1, and YY1 in MCF7 and HS578T cells.