Table 1 Immunohistochemical antibodies used to characterize the luminal cases
Protein (Clone) | Antibody | Origin | Clone | Pre-treatment | Dilution | Location of staining | Normal |
|---|---|---|---|---|---|---|---|
Androgen receptor (AR) | mmb | Dako | 4AR441 | PH9 Target retrieval solution (98°C, 40 min) | 1/50° | nucleus | + |
BCL2 | mmb | Dako | 124 | Citrate* | 1/100° | cytoplasm | + |
CyclinD1 CCND1 | mmb | Labvision | SP4 | EDTA* | prediluted | nucleus | + |
ERBB2 | mmb | Dako Herceptest Ltd | AO485 | Target retrieval * | 1/500 | membrane | - |
Estrogen receptor (ER) | mmb | Novocastra laboratories Ltd | 6F11.2 | Target retrieval * | 1/60 | nucleus | + |
FOXA1 | mmb | AbNova | 2D7 | Citrate* | 1/250 | nucleus | + |
GATA3 | mmb | Santacruz | HG3-31 | Citrate* | 1/100° | nucleus | + |
P53 | mmb | Beckmann | DO-1 | Citrate* | 1/4 | nucleus | - |
Progesterone receptor (PR) | mmb | Dako | PFR 636 | Target retrival * | 1/80 | nucleus | + |
Ki67 | mmb | Dako | KI-67 | Target retrieval solution * | 1/100 | nuclear | + |