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Figure 3 | Breast Cancer Research

Figure 3

From: Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis

Figure 3

IL-1β induces NFκB activation and cooperates with iFGFR1 to promote migration in HC-11/R1 cells. (a) HC-11/R1 cells were incubated in serum-free medium overnight and then stimulated with the following concentration of recombinant IL-1β for 15 minutes: 50, 5, 0.5 and 0.05 ng/ml IL-1β. Immunoblot analysis was performed to detect levels of phospho-p65 (upper panel) and total p65 (lower panel) as a loading control. (b) HC-11/R1 cells were treated with 5 ng/ml recombinant murine (rm) IL-1β for the indicated times and immunoblot analysis was performed as described in a. (c, d) At confluency, HC-11/R1 cells were serum starved for 24 hours, scratched using a pipet tip and allowed to recover for 18 hours in the presence of IL-1β, AP20187 or both. Pictures were taken immediately after the scratch and 18 hours later and were used to determine the extent of migration (d) measuring the changes in area between the scratch surfaces. Error bars represent standard error of the mean. ** P < 0.01, *** P < 0.001. iFGFR1 = inducible fibroblast growth factor receptor 1; NF = nuclear factor.

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