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Figure 1 | Breast Cancer Research

Figure 1

From: Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis

Figure 1

iFGFR1 activation induces IL-1β expression in the mammary gland. (a) Mouse mammary tumour virus (MMTV) inducible fibroblast growth factor receptor 1 (iFGFR1) transgenic mice and non-transgenic littermate controls were treated with AP20187 for 8 and/or 24 hours. Non-treated mice were used as the baseline control. Quantitative RT-PCR was performed on RNA extracted from whole mammary gland tissue and normalised to expression levels of cyclophilin. Mammary glands from three separate mice were analysed per time point. Error bars represent standard error of the mean. ** P < 0.01, *** P < 0.001. (b to e) Immunohistochemical analysis of mammary gland tissue sections from MMTV-iFGFR1 transgenic mice and non-transgenic littermate controls following either 48 hours or 4 weeks of AP20187 treatment. Images are representative of results from the analysis of three mice per genotype and treatment time. (f) Quantitative RT-PCR analysis of IL-1β expression levels (normalised to glyceraldehyde 3-phosphate dehydrogenase). Either HC-11/R1 cells or HC-11/R1-RAW264.7 co-cultures were treated with AP20187 (+AP) or solvent (-AP) for four hours and analysed for IL-1β gene expression. * P < 0.05. (g) HC-11/R1-RAW264.7 co-cultures were treated with AP20187 (+AP) or solvent (-AP) for 18 hours and conditioned media was analysed using an ELISA. * P < 0.05.

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