Figure 4From: Tamoxifen induces pleiotrophic changes in mammary stroma resulting in extracellular matrix that suppresses transformed phenotypesMammary extracellular matrix (ECM) proteolysis is reduced with tamoxifen treatment. (a) Western blot analyses of fibronectin, laminin 1 and laminin 5 in mammary tissue from control and tamoxifen-treated rats, with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control. (b) Fibrillar collagen detected by Western blot (left panel) and in 5 μm Picro-sirius red stained mammary gland sections. (c) Matrix metalloproteinase (MMP) 2 activity detected by zymography, with total MMP-2 levels and active MMP-2 levels quantified by scanning densitometry (*p = 0.005 and *p < 0.0001, respectively).Back to article page