Figure 2

Kaplan–Meier analysis of distant metastasis-free survival. (a) Analysis performed for 724 patients. In all cases except the Intrinsic/UNC subtyping gene set (HU) the blue (red) curve represents the good (poor) outcome group. Each panel depicts the result obtained after applying one of the classifiers (indicated in the heading) as described in Materials and methods. Represented signatures: T17 and T52, Molecular Prognostic Index signature [20]; IGS, Invasiveness Gene signature [16]; HU, Intrinsic/UNC gene set [15] – resulting in the Luminal A (blue), Normal-like (coral), Luminal B (red), Basal (grey) and Her2 (purple) subtypes – CSR, Core Serum Response signature [14]; CIN70 and CIN25, Chromosomal Instability signature [13]; GGI, Gene expression Grade Index [25]; and Wang, 76-gene signature [8]. (b) Top panel: performance of the signatures on subgroups of the patient population. Vertical axis, -log(P value) of the log-rank test from the Kaplan–Meier analysis for a particular subgroup with distant metastasis-free survival (DMFS) as the endpoint. Analyzed subgroups: lymph node (LN)-negative, LN-positive, estrogen receptor (ER)-positive, ER-negative, low grade (Elston Ellis I), high grade (Elston Ellis III), individual Chin, Desmedt, Loi and Minn datasets. Horizontal line, P = 0.05. Bottom panel: time-censoring performance analysis of the signatures. Horizontal axis, time at which right censoring was applied to all samples; vertical axis, -log(P value) of the log-rank test from the Kaplan–Meier analysis for a given time-censoring and a particular signature with DMFS as the endpoint. Horizontal line, P = 0.05.