Figure 4

Quantification of fluorochrome accumulation in a double xenografted mouse by FMT. (a-c) Fluorescence-mediated tomography (FMT) was performed 24 hours after injection of SIDAG in order to resolve the three-dimensional fluorochrome distribution and for quantification of fluorochrome concentration in the target tissue: panel a shows a white light image, panel b shows colour-coded FMT data for MDA-MB435, and panel c shows the DU4475 xenograft superimposed on white light images. The average fluorochrome concentration in the MDA-MB xenografts ranged around 229 ± 90 nmol/l, whereas DU4475 exhibited significantly lesser fluorochrome accumulation (49 ± 22 nmol/l; P < 0.01).