Sample | Antibody/antibodies | ToPRO-3 or DAPI | ||
---|---|---|---|---|
 | First incubation | Second incubation | Third incubation |  |
Nonspecific staining control | Rat immunoglobulin | α-rat-FITC | IgG-PE IgG-PE-Cy5 2IgG-PE-Cy7 | No |
ToPRO-3 or DAPI control | None | N/A | N/A | Yes |
33A10 control | 33A10 | Anti-rat-FITC | N/A | No |
Sca-1 control | α-Sca-1-PE | N/A | N/A | No |
CD45 control | aα-CD45-PE-Cy5 or bα-CD45-PE-Cy7 | N/A | N/A | No |
bCD24 control | bα-CD24-PE-Cy5 | N/A | N/A | No |
Experimental sample | 33A10 | α-rat-FITC | α-Sca-1-PE aα-CD45-PE-Cy5 or bα-CD45-PE-Cy7 bα-CD24-PE-Cy5 | Yes |