Table 1 Primer sequences and temperature conditions for real-time quantification analysis
From: Circulating Bmi-1 mRNA as a possible prognostic factor for advanced breast cancer patients
Primer | Sequence | Annealing temperature (°C) |
|---|---|---|
BmiQ-F | GCTTCAAGATGGCCGCTTG | 65 |
BmiQ-R | TTCTCGTTGTTCGATGCATTTC | |
BmiQ-F2 | CATTGTCTTTTCCGCCCGC | 63 |
BmiQ-R2 | CAAAGCACACACATCAGGTGGG | |
UbcQ-F | ATTTGGGTCGCGGTTCTTG | 59 |
UbcQ-R | TGCCTTGACATTCTCGATGGT |