Figure 5

Endothelial cell migration. G3-containing medium enhances endothelial cell migration 48 h following gap formation (a). Monolayer endothelial cell cultures were wounded (0 h) by micro-tips and washed with PBS, followed by addition of culture medium pre-incubated with G3- and vector-transfected cells. Addition of G3-containing medium enhanced endothelial cell migration to the wounding areas. Endothelial cell migration is enhanced in the presence of purified G3, fibronectin (FN) and VEGF (b). Endothelial cells were seeded on 12-well tissue culture plates. The cultures were wounded with micro-tips (before) and washed, and fibronectin, VEGF and purified G3 were added individually or in combination as indicated. In the presence of all three molecules, cells exhibited enhanced migratory capacity. Medium represents culture media in the absence of G3, VEGF, and FN.