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Table 2 Effect of Wilms' tumor 1 suppressor gene (WT1) plasmid with ZF - KTS on the growth of breast cancer cells

From: The zinc finger domain of Wilms' tumor 1 suppressor gene (WT1) behaves as a dominant negative, leading to abrogation of WT1 oncogenic potential in breast cancer cells

Cells pcDNA3 pcDNA3/ZF - KTS pcDNA3/WT1 pSuper pSuper/WT1
MCF-7      
   Mean ± standard deviation (CFU) 142 ± 3.3 51 ± 4* 164 ± 9* 51 ± 4.8 40 ± 3.7*
   Relative change (%) 0 -64.1 +15.5 0 -21.8
MDA468      
   Mean ± standard deviation (CFU) 291 ± 8.7 214 ± 15.3* 406 ± 17* 177 ± 6.1 42 ± 1.7*
   Relative change (%) 0 -26.5 +39.5 0 -76.2
MDA231      
   Mean ± standard deviation (CFU) 131 ± 6.2 130 ± 3.8 142 ± 5 36 ± 1.1 32 ± 3.0
   Relative change (%) 0 -0.2 +8.8 0 -11.2
  1. Relative change represents the percentage difference in number of colony-forming units (CFU) produced in the zinc finger domain lacking the lysine–threonine–serine insert (ZF - KTS), in full-length WT1 or in WT1 shRNA transfected cells in comparison with empty vector, respectively. *P < 0.05.