HMEC strain | No treatment | E2 | E2 + fulvestrant |
|---|
AJ | 0/14 | 10/10 | 1/8 |
AK | 0/14 | 12/12 | 3/6 |
U | 0/4 | 11/12 | 0/8 |
Total | 0/32 | 31/32 | 4/22 |
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ERα/BMI1/TERT/MYC-transduced HMECs from three donors (AJ, AK and U) were injected into the inguinal mammary glands of NOD/SCID mice. Mice were divided into three groups: the control group (no treatment), which received no additional drugs (the cells were therefore exposed only to endogenous mouse oestrogen); the oestrogen-treated group, which received slow-release oestrogen pellets containing 1.5 mg of oestradiol (E2); and the fulvestrant-treated group, which received oestrogen pellets to allow engraftment followed after 32 days by weekly injections of fulvestrant to block oestrogen stimulation (E2 + fulvestrant). ER, oestrogen receptor; HMEC, human mammary epithelial cell.
