Figure 6

Keratin expression by tumours at different time points. The top panels show haematoxylin/eosin staining of tumours harvested at (a) 14 days, (b) 35 days and (c) 60 days after injection of oestrogen receptor alpha (ERα)/BMI1/TERT/MYC/cyan fluorescent protein (CFP)-transduced human mammary epithelial cells (HMECs). The lower panels show matched sections immunostained for keratins. (d-f), Keratin 14 (green); (g-i), keratin 18 (red); (j-l), merged image of the keratin 14 and keratin 18 signals together with a nuclear counterstain (4',6-diamidino-2-phenylindole (DAPI), blue).