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Figure 3 | Breast Cancer Research

Figure 3

From: Stat3 is tyrosine-phosphorylated through the interleukin-6/glycoprotein 130/Janus kinase pathway in breast cancer

Figure 3

Signal transducer and activator of transcription 3 (Stat3) phosphorylation is mediated through Janus kinases (Jaks) but not epidermal growth factor receptor (EGFR) or Src kinases. (a) Radioimmunoprecipitation assay (RIPA) extracts (50 μg) isolated from MDA-MB-468 cells treated for 4 hours with dimethyl sulfoxide (DMSO), P6 1 μM, BMS 50 nM, and ZD 2 μM were analyzed for pEGFR, total EGFR, tyrosine-phosphorylated Stat3 (pStat3), Stat3, pSrc, Src, pJak2, and Jak2. (b) Nuclear extracts (20 μg) isolated from MDA-MB-435 cells (upper panels) and RIPA extracts (bottom panels) treated as in (a) and probed with the indicated antibodies. (c) Nuclear extracts (20 μg) isolated from MDA-MB-231 cells (upper panels) and RIPA extracts (bottom panels) treated as in (a) and probed with the indicated antibodies. (d) Ten thousand cells (MCF10A, MCF7, MDA-MB-468, and MDA-MB-435) were plated into a 96-well culture dish and treated with DMSO control or P6 (1 μM) for 48 hours, and proliferation was measured using an MTT (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay.

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