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Figure 7 | Breast Cancer Research

Figure 7

From: Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors

Figure 7

The effect of fibroblast-conditioned media on PMC42-LA organoid morphology in three-dimensional cultures. CAF-conditioned medium was added to or below PMC42-LA filter cultures, and the cultures analysed for changes in organoid morphology possibly representative of increased invasiveness. Controls had no fibroblast conditioned-medium. (a,b) In control cultures, organoids appeared spherical with little, if any, single cells present. (c,d) With CAF-conditioned media on the filter/culture, organoids remained predominantly spherical, with some budding edges and the presence of single cells and clusters of single cells. (e,f) With CAF-conditioned medium below the filter/culture, organoids appeared less spherical, with uneven budding edges and many single cells and clusters of single cells. CAF, cancer-associated fibroblast.

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