DCs loaded with killed breast cancer cells induce differentiation of naïve CD8+ T cells into effector cells. Sorted naïve CD8+ T were cultured for 3 weeks with autologous DCs either (a) not loaded or (b) loaded with killed breast cancer cells. The cultures were restimulated twice. Intracytoplasmic staining and flow cytometry are given for the indicated effector molecules. Shown is one representative experiment from three with T cells from three different healthy donors. Percentage of CD8+ T cells expressing (c) indicated cytokines or (d) effector molecules in cultures with unloaded (black bars) and loaded (gray bars) DCs. DC, dendritic cell; GM-CSF, granulocyte-macrophage colony-stimulating factor; Gr, granzyme; IFN, interferon; IL, interleukin.