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Figure 2 | Breast Cancer Research

Figure 2

From: Characterization of tamoxifen and 4-hydroxytamoxifen glucuronidation by human UGT1A4 variants

Figure 2

Representative mass spectra of trans-4-hydroxytamoxifen quaternary ammonium glucuronide formed by human liver microsomes. The predicted trans-4-hydroxytamoxifen quaternary ammonium glucuronide (trans-4-OH-TAM-N +-glucuronide) was collected after separation by HPLC and identified by liquid chromatography–mass spectrometry using a Shimadzu LC-MS 2010 EV system. trans-4-OH-TAM-N +-glucuronide was loaded onto a C18 Shimadzu reverse-phase column and analyzed at a flow rate of 0.2 ml/min by applying a linear mobile phase gradient from 10% to 80% (v/v) methanol/H2O over 30 min. An electrospray voltage of 1.5 kV was applied using a positive mode.

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