Figure 3

RT-PCR and sequencing analyses of partial extracellular domain of rat neu in the tumor-derived cell lines. (a) RT-PCR analyses of rat neu. Total RNA isolated from the indicated tumor cells by the TRIZOL reagent was analyzed with electrophoresis using a 1% agarose gel containing ethidium bromide and visualized under UV light (bottom). First-strand cDNA was synthesized using a kit from Roche Diagnostics Corp. The partial extracellular domain of rat neu was amplified with specific primers. The PCR products were separated on a 1.2% agarose gel containing ethidium bromide and visualized under UV light (top). (b) Partial sequencing of the PCR product from 83923 cells with reverse primer AB1310. The mixture of wt nucleotide T (red) and mutant nucleotide A (green) is indicated by an arrow.