Figure 4From: Somatic mutation and gain of copy number of PIK3CA in human breast cancerBiological effect of PIK3CA mutations in breast cancer. (a) Phosphorylation (P) of Akt in breast cancer cells. Western blotting showed stronger phosphorylation of Akt in BT20 and MCF7 cells than in MDA 231, MDA361, BT474, or T47D. β-Actin was used as a protein loading control. (b) Summary of the fraction of apoptotic cell in three cell lines with different treatments (3 μM and 10 μM LY294002). [(c) Apoptosis measurement in which cells were stained with Annexin V-FITC and for DNA content with Propidium Iodide and analyzed using flow cytometry. Apoptotic cells appear as a discrete population with elevated FITC fluorescence. FITC, fluorescein isothiocyanate; SS, serum starvationBack to article page