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Figure 1 | Breast Cancer Research

Figure 1

From: Differential responses to doxorubicin-induced phosphorylation and activation of Akt in human breast cancer cells

Figure 1

Differential responses to doxorubicin-induced phosphorylation of Akt in a panel of human breast cancer cells. (a) MCF7 cells were exposed to 1 μM doxorubicin (Doxo) for the indicated periods in culture medium supplemented with 10% fetal bovine serum (FBS). The cells were then harvested and prepared for Western blot analyses with antibodies directed against Ser473-phosphorylated Akt (p-Akt) and total Akt. The densitometric levels of p-Akt at each time point were normalized to the corresponding levels of total Akt and are shown in the bar graph. (b) A panel of eight indicated breast cancer cell lines were treated with 0.125 to 1 μM doxorubicin for 24 hours in culture medium supplemented with 10% FBS. After treatment the individual cell lines were harvested, lysed and subjected to Western blot analyses with antibodies against p-Akt and total Akt.

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