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  • Poster Presentation
  • Open Access

Chromosomal imbalances mapped by array-based comparative genomic hybridization in an integrated approach to combat breast cancer in Denmark

  • 1,
  • 1,
  • 1,
  • 1,
  • 1 and
  • 1
Breast Cancer Research20057 (Suppl 2) :P4.37

https://doi.org/10.1186/bcr1167

  • Published:

Keywords

  • Breast Cancer
  • Comparative Genomic Hybridization
  • Substantial Admixture
  • Breast Cancer Sample
  • Breast Cancer Research

Since its invention by Kallioniemi and colleagues in 1992 [1], comparative genomic hybridization (CGH) has revolutionized the detection and mapping of chromosomal imbalances in neoplasias. However, conventional CGH is handicapped by its low resolution. Array-based CGH brings the resolution towards a molecular level. With a capillary printer we produce arrays on CodeLink slides with 3158 BAC clones, which randomly represent the whole genome. With our homemade arrays, we can detect and map numerical aberrations in a single experiment with about 1 Mb resolution. Furthermore, we have optimized printing, labeling, hybridization, scanning and analysis tools. Reverse-labeling (exchanging the tumor and reference DNA labeling dyes) gives us reliable results even in samples with a substantial admixture of normal cells.

In the Danish Centre for Translational Breast Cancer Research, a 5-year project involving 500 high-risk patients is underway. Both prospective and retrospective studies are planned with a systems biological approach involving a multitude of analyses, including array-CGH. Twenty breast cancer samples have been analyzed in a preliminary study. Chromosome 1q (15/20), chromosome 8 (14/20), chromosome 11 (5/20), chromosome 17q (9/20) and chromosome 20q (6/20) gains (duplications and amplifications), and chromosome 22 (7/20) deletions are the most frequent aberrations, which is consistent with the previously published conventional CGH results [2]. Our findings will continuously be integrated with all the other results from the same tumors.

Authors’ Affiliations

(1)
Institute of Human Genetics, University of Aarhus, Denmark

References

  1. Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D: Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992, 258: 818-821.View ArticlePubMedGoogle Scholar
  2. Stephanie S, Martine DF, Pascale CL: Compilation of published comparative genomic hybridization studies. Cancer Genet Cytogenet. 2002, 135: 63-90. 10.1016/S0165-4608(01)00624-0.View ArticleGoogle Scholar

Copyright

© BioMed Central 2005

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