Table 1 Primers used to amplify exons and splice junctions of ATR
From: Mutation analysis of the ATRgene in breast and ovarian cancer families
Exon | Forwarda | Reverse |
|---|---|---|
1 | ccgggtcctatgcagaaaag | aggggagagcacgtgaaac |
2 | cattgacactgaacacatttgatg | tctaaaactacatggagaaaatgct |
3 | ggcccacagtctggtttct | gtaatatttcagaagagcagtaaaagg |
4a | tcgtcaaggatttagcaaatga | acgagtaagaaccattaataaagtgac |
4b | atgtgatgggtcatgctgtg | gctcttcatagagtttcaattggtc |
4c | tccaaaaattaaatccctagcaa | tctcacatagaccttcctgacttg |
4d | aaactctgtgtcatgtttgaagac | gccagactacactatgaaaatcatta |
5 | cattcttgctgcctatgaataa | aaatcaaagcacttaactaaagctga |
6 | tctaaatatgtttcatgttttaaccaa | tgagtcaagtgaataatgagtaaaca |
7 | tggctttactacaattttatgtttgac | cacttaggcttcaggcaaaa |
8b | tttaaaagagatatgattaaggaaaag | cacacattcttgtgagcactt |
9 | aaatgtattttaagtgttacttgactttt | aaccctgcatacatagccaga |
10 | gtcccaaattaagcaagactattt | caaggcttcagtctaattcttttac |
11 | tcatggcatattatttgttgac | gaacaataaaattaactggttaaagaa |
12 | gaggttgataatttttgtttttaacat | ccatttttaacagcaagcaaa |
13 | tgagtcaacatgaatttatttgtagat | aaagaaaagcaagcaaaataaaac |
14 | ctctatggtggcttaaaaagtattagt | caaagtcaaaatctagaatggaatg |
15 | ctccaaatatgtgtggcattt | accctctttcctagaagaatgttac |
16 | ctcctgatgtactaatagcatgttaaa | tgaccaaaaatatgatttcttcaat |
17 | gcttttggagaaacttaattaacca | tgtttgtagctagatgcagaattt |
18b | tgtccttagggctcatctgc | tgaacccaatttccctcaaa |
19 | gctgccttttaatctattgtttg | cattaccatcagtaattttgagacat |
20 | ggccttagtttcaacttttactttaca | caggaattagctatcagaataggact |
21 | gagaattcaggcctttggaa | aatgtcattttgtcatcttttcttt |
22 | aactcatcaaaaactagctgaaaaa | ggataagctgaatagttctttgtaaat |
23 | ccatggaaaaagcagtacacc | aaaacaaaaaggagtttcacaagt |
24 | gcataaataaagcgaagtgcaa | ggccaaaaaaatcgcatta |
25 | agtcaactgaaggagttgctg | ttgtgtgtgctaggcattcag |
26 | ttatctcacatgctactctttgaca | catttcctactaataggtagcctttc |
27 | ttagaatggttagctttagatgtcata | agaactgataaagggaagagctaa |
28 | caattgttctgttgttagttacattct | gcatagcatataaaacattcaataaaa |
29 | aaacaggtggttttatagttttatttc | aaggtttccagagttcctattca |
30 | aagggcaataaggtaaatagtaat | aaattacccaattcactaactaaaaa |
31 | gaacaaaatacaatataatgcaaattcaa | accgcacccatcctaaaact |
32 | ttgatatttcagctgaccattttatc | ccaaactcactatcaattatttactcaaa |
33 | caactgtgtattttaaattctttatttctg | cacccccaaataatatccaa |
34b | attgggaacagaggctttca | gacatttccctggccattac |
35 | caaaaacataatgaactaatacttttgc | catgtgctttgccatatagactt |
36 | tcacatacttttgatccctaatca | acctagaatatgctaagacatgtga |
37 | tttttgtgaaaacggtatgtgg | agactgtccagccaaatctga |
38 | tgtgaaatgaactgatatactgattttt | cgccctggaacttgtatcta |
39 | aactctcatcatgaatactttttaagtt | aaaaactgctttattaagacaaatcat |
40 | ttgtaaaagtgaaatttttgttatagtgg | ttgtgaaatacactttttatcttaatttga |
41 | tttacacagaaatttttggcccta | caactctgaaataaaagcaatctgg |
42 | tttggttatgaaatgaacaatcttt | aggaagggatggaaacactt |
43 | agtagatgtttcttgtccaattttaac | catatgaggccaatataaatctaaaa |
44 | gttgttatggttgaatgtttattttta | caaggaagatacagttgttgagaa |
45 | tggacatgaagttctttgagtaaa | caaacatatgtaggggccaat |
46 | agcttctcatccttcacttaaa | aactatagctgcatatcaagttca |
47a | gggtattggtcagtaaaatggta | ccacagattcataccaaatgc |
47b | gaaggacatgtgcattaccttatac | cttgcttgtttcttgcaaatatag |