Figure 6

Celecoxib treatment causes reduction in microvascular channel formation by regulating VEGF levels. (a) The percentage of cells forming channels was much greater in MDA-MB-231 cells than in MDA-MB-468 cells. In both cells, treatment with 40 and 60 μmol/l celecoxib caused significant reduction in the number of channels. P values represent significant differences between vehicle control and celecoxib treatment. (b) Western blot analysis of cell lysates prepared from vehicle and celecoxib (20–60 μmol/l) treated cells. SDS-PAGE electrophoresis was performed using 15% resolving gel. Protein was loaded at 100 μg per lane and the protein was detected using vascular endothelial growth factor (VEGF) antibody. Celecoxib treatment decreased VEGF levels in MDA-MB-231 cells in a dose-dependent manner.