Muscarinic receptors participation in angiogenic response induced by macrophages from mammary adenocarcinoma-bearing mice

Table 1 Participation of mAchR subtypes in angiogenesis induced by TMps

Treatment	Angiogenic response (vessels/mm²)	n
None	2.33 ± 0.07	5
CARB	4.98 ± 0.40**	5
AT + CARB	2.57 ± 0.25	5
PIR + CARB	2.49 ± 0.63	4
MET + CARB	3.35 ± 0.17*	5
4-DAMP + CARB	2.64 ± 0.48	6

Peritoneal macrophages (3 × 10⁵) from 7-day LMM3 tumor-bearing mice (TMps) untreated or stimulated with carbachol (CARB; 100 nM) in the absence or presence of 1 μM atropine (AT), pirenzepine (PIR), methoctramine (MET) or 4-diphenylacetoxy-N-methylpiperidine (4-DAMP) were inoculated intradermally into normal mice to evaluate the angiogenic response. **P < 0.0001, *P < 0.05 compared with untreated TMps (Mann–Whitney test). n, number of sites evaluated. Results are means ± SEM for three experiments.

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