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Figure 2 | Breast Cancer Research

Figure 2

From: Muscarinic receptors participation in angiogenic response induced by macrophages from mammary adenocarcinoma-bearing mice

Figure 2

Cyclo-oxygenase (COX) muscarinic receptors in TMps. Upper panel: macrophages from 7-day LMM3 tumor-bearing mice (TMps) were treated with carbachol (CARB) (100 nM) in the absence or presence of 1 μM indomethacin (INDO) 10 μM NS-398, 1 μM atropine (AT) or 4-DAMP. COX activity was measured by prostaglandin E2 (PGE2) liberation by TMps as pg/106 cells. Values are means ± SEM for three experiments. *P < 0.001 compared with basal; # P < 0.001 compared with CARB; NS, not significantly different from basal by Tukey's modified t-test. Lower panel: Western blot assay to detect COX isoforms and muscarinic receptors (M) in lysates of TMps. Molecular masses of the bands indicated on the left are coincident with COX-1, COX-2 and M3 proteins. One representative experiment of three is shown.

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