- Paper Report
- Open Access
Cyclin D1in breast cancer is not associated with Ki67 but with ER
- George Skliris1
© Biomed Central Ltd 2001
- Received: 30 July 2001
- Accepted: 20 August 2001
- Published: 1 December 2001
- Cancer, cyclin D1, immunohistochemistry, Ki67, normal breast, oestrogen receptor
D-type cyclins are implicated in cell cycle regulation. The cyclin D1 gene is amplified in 15-20% of breast cancers while the protein is overexpressed in 50% of these cancers (see Additional information ). Overexpression of cyclin D1 has been linked with oestrogen receptor (ER)a, because it can stimulate transcriptional functions in the absence of oestrogen (see Additional information ). A negative association in normal breast has been reported for ERa and Ki67 (a proliferation marker), although this relationship changes in many precancerous and ER-positive cancers. Therefore, the aim of the study was to investigate the interaction between cyclin D1, ER and Ki67 in normal and malignant breast material.
The mean percentage of ER-positive cells in normal breast tissues was 20%. Approximately 50% of the cyclin-D1-positive cells coexpressed ER. However, cyclin-D1-positive cells were detected in 70% of ER-positive invasive ductal carcinomas (IDCs) and in 30% of ER-negative IDCs. In normal breast tissues, Ki67-positive cells accounted for 3% of the epithelial cells while only 0.3% contained cyclin D1. In benign breast lesions no clear outcome was detected for cyclin D1 and Ki67. Invasive cancers showed a higher percentage of Ki67-positive cells than normal tissue and this was significantly higher in ER-negative than in ER-positive carcinomas. Overall a positive correlation between cyclin D1 and ER-positive cells was detected in normal and cancerous breast tissues, while a negative association was detected between cyclin D1 and Ki67.
Dual immunofluorescence immunohistochemistry
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